Scientist/Senior Scientist, Synthetic Biology
Pattern Biosciences
South San Francisco, CA
www.patternbio.com
About the Company
Do you want to be at the forefront of a revolution in healthcare? At Pattern, we build DNA computers that run molecular programs in cells to treat cancer and other diseases. Using multi-input molecular-level computation in individual cells, our approach leads to curative therapies where the conventional one drug-one target paradigm has failed. Pattern is building a world-class team to translate two decades of progress in DNA computing and synthetic biology into the clinic.
About the Role
Are you passionate about working with cutting-edge technologies to develop next generation therapies? You will advance the early pipeline of innovative cancer therapies harnessing our biomolecular computing platform. You will engineer genetic scaffolds to compute across multiple molecular inputs and program cancer cells for destruction with exquisite specificity. You will focus on individual sensor components, molecular computing scaffolds, and fully assembled therapeutic leads.
What you'll do
- Design and construct barcoded genetic component libraries, logic scaffolds, effectors, and product leads
- Characterize component libraries and individual therapeutic candidates as naked and vectorized DNA in mammalian cell culture and in vivo specimens. You will use next-generation sequencing, fluorescent microscopy, flow cytometry, qPCR, and plate reader assays
- Develop and/or implement novel assay systems for improved characterization of genetic components
- Map the relationship between naked and vectorized DNA performance and assess the impact of DNA sequence on viral vector manufacturing
- Work closely with the Computational Biology and In Vivo Teams to plan and execute experiments that inform the design-build-test cycle of components and therapeutic leads
Requirements
- PhD in molecular biology, genomics, bioengineering or a related field
- Excellent knowledge of gene regulatory and gene expression processes and mechanisms in human cells, and the ability to translate this knowledge into actionable genetic designs
- Deep knowledge in experimental planning and execution of high-throughput cloning of barcoded libraries and individual genetic constructs using “Golden Gate”, Gibson assembly, yeast homologous recombination, restriction-ligation
- Experience in mammalian cell culture, fluorescent microscopy, flow cytometry, cell sorting, plate reader assays including ELISA, and RT-qPCR assays
- Hands-on experience in library preparation for next-generation sequencing
- Manufacturing of Adeno-Associated virus (AAV) vectors
- Preferably, prior experience in animal experimentation
Benefits
- Health, dental and vision insurance
- 401(k) program
- Snacks provided
- Gym on-site
- Easy parking and access to EV charging
This is an in-person role based at our headquarters in South San Francisco, CA. To apply, please send a cover letter and your resume to careers@patternbio.com.